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Rg3 alleviated cage stress induced liver injury by suppressing inflammation and promoting autophagy. (A) Volcano plot (Rg3-H vs. ST); expression of inflammation-related genes in the liver of laying duck; IF of F4/80 protein in the liver of laying ducks. (B-C) Volcano plot (Rg3-H vs. ST); expression of antioxidant-related genes in the liver of laying duck; IF of GPX4 and ACSL4 protein in the liver of laying duck. (D) Volcano plot (Rg3-H vs. ST); expression of autophagy-related genes in the liver of laying duck; IF of <t>LC3B</t> protein in the liver of laying duck. Data are presented as the mean ± SEM; Intergroup differences were marked with lowercase letters. Different letters indicate statistically significant differences between groups (P < 0.05).
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Rg3 alleviated cage stress induced liver injury by suppressing inflammation and promoting autophagy. (A) Volcano plot (Rg3-H vs. ST); expression of inflammation-related genes in the liver of laying duck; IF of F4/80 protein in the liver of laying ducks. (B-C) Volcano plot (Rg3-H vs. ST); expression of antioxidant-related genes in the liver of laying duck; IF of GPX4 and ACSL4 protein in the liver of laying duck. (D) Volcano plot (Rg3-H vs. ST); expression of autophagy-related genes in the liver of laying duck; IF of <t>LC3B</t> protein in the liver of laying duck. Data are presented as the mean ± SEM; Intergroup differences were marked with lowercase letters. Different letters indicate statistically significant differences between groups (P < 0.05).
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Rg3 alleviated cage stress induced liver injury by suppressing inflammation and promoting autophagy. (A) Volcano plot (Rg3-H vs. ST); expression of inflammation-related genes in the liver of laying duck; IF of F4/80 protein in the liver of laying ducks. (B-C) Volcano plot (Rg3-H vs. ST); expression of antioxidant-related genes in the liver of laying duck; IF of GPX4 and ACSL4 protein in the liver of laying duck. (D) Volcano plot (Rg3-H vs. ST); expression of autophagy-related genes in the liver of laying duck; IF of <t>LC3B</t> protein in the liver of laying duck. Data are presented as the mean ± SEM; Intergroup differences were marked with lowercase letters. Different letters indicate statistically significant differences between groups (P < 0.05).
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Rg3 alleviated cage stress induced liver injury by suppressing inflammation and promoting autophagy. (A) Volcano plot (Rg3-H vs. ST); expression of inflammation-related genes in the liver of laying duck; IF of F4/80 protein in the liver of laying ducks. (B-C) Volcano plot (Rg3-H vs. ST); expression of antioxidant-related genes in the liver of laying duck; IF of GPX4 and ACSL4 protein in the liver of laying duck. (D) Volcano plot (Rg3-H vs. ST); expression of autophagy-related genes in the liver of laying duck; IF of <t>LC3B</t> protein in the liver of laying duck. Data are presented as the mean ± SEM; Intergroup differences were marked with lowercase letters. Different letters indicate statistically significant differences between groups (P < 0.05).
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Rg3 alleviated cage stress induced liver injury by suppressing inflammation and promoting autophagy. (A) Volcano plot (Rg3-H vs. ST); expression of inflammation-related genes in the liver of laying duck; IF of F4/80 protein in the liver of laying ducks. (B-C) Volcano plot (Rg3-H vs. ST); expression of antioxidant-related genes in the liver of laying duck; IF of GPX4 and ACSL4 protein in the liver of laying duck. (D) Volcano plot (Rg3-H vs. ST); expression of autophagy-related genes in the liver of laying duck; IF of <t>LC3B</t> protein in the liver of laying duck. Data are presented as the mean ± SEM; Intergroup differences were marked with lowercase letters. Different letters indicate statistically significant differences between groups (P < 0.05).
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Rg3 alleviated cage stress induced liver injury by suppressing inflammation and promoting autophagy. (A) Volcano plot (Rg3-H vs. ST); expression of inflammation-related genes in the liver of laying duck; IF of F4/80 protein in the liver of laying ducks. (B-C) Volcano plot (Rg3-H vs. ST); expression of antioxidant-related genes in the liver of laying duck; IF of GPX4 and ACSL4 protein in the liver of laying duck. (D) Volcano plot (Rg3-H vs. ST); expression of autophagy-related genes in the liver of laying duck; IF of LC3B protein in the liver of laying duck. Data are presented as the mean ± SEM; Intergroup differences were marked with lowercase letters. Different letters indicate statistically significant differences between groups (P < 0.05).

Journal: Poultry Science

Article Title: Ginsenoside 20(S)-Rg3 alleviates cage stress induced liver injury in laying ducks

doi: 10.1016/j.psj.2025.106254

Figure Lengend Snippet: Rg3 alleviated cage stress induced liver injury by suppressing inflammation and promoting autophagy. (A) Volcano plot (Rg3-H vs. ST); expression of inflammation-related genes in the liver of laying duck; IF of F4/80 protein in the liver of laying ducks. (B-C) Volcano plot (Rg3-H vs. ST); expression of antioxidant-related genes in the liver of laying duck; IF of GPX4 and ACSL4 protein in the liver of laying duck. (D) Volcano plot (Rg3-H vs. ST); expression of autophagy-related genes in the liver of laying duck; IF of LC3B protein in the liver of laying duck. Data are presented as the mean ± SEM; Intergroup differences were marked with lowercase letters. Different letters indicate statistically significant differences between groups (P < 0.05).

Article Snippet: Subsequently, 5 % bovine serum albumin (BSA) was added for blocking at room temperature for 1 h. Primary antibodies against F4/80 (28463-1-AP, Proteintech), GPX4 (30388-1-AP, Proteintech), ACSL4 (sc-365230, Santa Cruz), and LC3B (14600-1-AP, Proteintech) were added, and the sections were incubated overnight at 4°C.

Techniques: Expressing